Located in UAB's Biomedical Research Building II (BMRII), the Creighton Lab is an 800 sq. ft. contiguous laboratory space. Within the laboratory are cell isolation and culture facilities, a dedicated microscope room, a rodent surgical suite, and separate room for recombinant DNA work.
Techniques we use in the lab:
- Cell isolation, characterization, and culture – special focus on endothelial cells. We work with cells from humans, rats, mice, knockout animals, and disease models.
- Environmentally regulated cell studies: light and fluorescence microscopy
- Microscopy: fluorescent, video, time lapse, histological
- Permeabilit: Ohm measurements, and labeled tracers
- Protein and Molecular Biology: PCR, Westerns, ELISA
- Spectroscopy: absorbance, fluorescence, luminescence, FACs
- Electric Cell Impedance Sensing (ECIS): resistance and capacitance determined under static and flow conditions
- Surgical: lung physiology-PCO2, PO2, Kf, pH, compliance
- Histology: specimen preparation, staining , immunohistochemistry
- Genetics: adenoviral, retroviral, and lentiviral mediated protein overexpression, chimera (fusion) proteins, and knock down (shRNA) . Stable transfections.