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| nuclear spectrin PDE 4D4 merge |
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Fluorescent images of lung tissue taken with a NikonTi85 microscope. The second and third row of each panel are close-up sections taken from the top row (indicated in the boxes). Blue, nuclear stain. Red, fluorescent tagged antibody to non-erythroid spectrin. Green, fluorescent tagged antibody to type 4D4 phosphodiesterase. Merge, overlay of the different images. |
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AMPK Activation Using AICAR Restores |
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Rate of gap resealing is given in microns/sec. Velocity determined using time lapse video microscopy and Nikon Instruments moving object software. Control velocity 6.63 x 10-3+/- 0.46 x 10-3 µm/s, LPS velocity 4.44 x 10-3 +/- 1.22 x 10-3 µm/s, LPS + AICAR velocity 7.30 x 10-3 +/- 1.41 x 10-3 µm/s. |