Explore UAB

The C2+ is designed as an essential microscopy tool for the laboratory, providing powerful and robust imaging capabilities. The high-efficiency scan heads and detectors, coupled with Nikon’s unrivaled optics, provide superior confocal images. The high-speed galvano scanners, operating at rates of up to 100 fps*, enable even the fast-beating motion of cardiac muscles to be captured with precision. The system has three simultaneous channels with an additional Transmitted light channel. Also a tunable variable bandwidth 32 channel spectral detector for research that requires spectra Imaging in addition to the standard detector.

Features:

• RGB and Far-Red imaging capabilities with High-Definition Diascopic DIC images
• C2-DUVB High Sensitivity Spectal Detection: 32 Channel Enhanced Spectral Detection with two GaAsP detectors and Virtual Filter option for customizable emission settings. Spectral Un-mixing
• Multiple wavelength gratings for Lamba scans (2.5nm/5nm/10nm steps)
• High speed time-lapse at up too 100fps
• Increased High detection sensitivity for photosensitive samples with GaAsP detection
• 3D and 2D imaging capabilities
• Spectral FRET Analysis

Objectives:

• SR HP Apo Tirf 100x AC na 1.5 wd 120
• 60x Apo Tirf 60x oil DIC n2 na 1.5 wd 120um
• 20x plan Apo vc 20x DIC n2 na o.8 wd 1000um
• Plan Fluor 40x Oil DIC H N2
• Plan Apo 60x Oil λS DIC N2
• Plan Fluor 100X 1.49 NA, Oil, DIC

LU-NV Laser Launch System:

405nm, 488nm, 561nm, 647nm

Accessories:

• Perfect Focus System
• Nikon Eclipse Ti2 inverted microscope
• Motorized x-y stage with encoders
• Piezo Z-stage
• Tokai Hit Environmental Chamber

Acquisition Software:

The Nis Elements 5.0 Imaging Software contains a cutting-edge analysis package of modules for doing quantification and densitometry of fluorescence in 2D and 3D.  Nis Elements can also be used to count cells and track cells on time-lapse movies.

Features:

• Four highly sensitive Power HyD® S detectors.
• Acousto Optical Beam Splitter (AOBS®).
• Exclusive White Light Laser that can accommodate any excitation wavelength between 485 nm and 790 nm.
• TauSense® technology allows for extended detection range (up to 850 nm).
• Up to 8 simultaneous excitation wavelengths.
• Imaging spectrally overlapping fluorophores with advanced separation of fluorescent signal.
• Access to functional information (metabolic status, pH, ion concentration).
• Super-resolution (down to 120nm) with adaptive LIGHTNING® detection.
• Fluorescent Lifetime Imaging Microscopy (FLIM).

Objectives:

• HC PL APO CS2 63x/1.40 OIL
• HC PL APO CS2 20x/0.75 IMM
• HC PL APO 20x/0.70 DRY
• HC PL APO CS2 10x/0.40 DRY
• HC PL FLUOTAR 5x/0.15 DRY
• HCX PL FLUOTAR,1.6x/0.05 DRY

Accessories:

• Motorized x-y-z stage
• Leica autofocus (only for live or samples in liquid media)

Features:

• Nikon TE2000 inverted microscope
• Prior Scientific motorized XY stage
• Multiple region time lapse with z-stack for 3D imaging. Timelapse and Spectral detection can be done on the A1 as well as Fluorescence Recovery After Photobleaching (FRAP) experiments. With the addition of a resonance scanner the A1 can image frames at over 300fps!  It can easily be switched from standard galvo scanning to Resonance or Spectral mode within the Nis Elements software.
• Nikon Perfect Focus
• Photo-manipulation (photo-bleaching/photo-activation) experiments can be easily conducted with multiple user-defined regions of interest
• Tandem galvano and resonant scanner up to 30 512X512 images/sec
• Advanced Detection: 4 PMT detectors and a transmitted light detector The detection system in the A1 Scanner consists of a highly sensitive array of PMT's which are capable of integrating line scans at a faster rate than previous confocals were able to accomplish making the system ideal for imaging large amounts of data.

Objectives:

• Plan Apo λ 4x dry
• Plan Fluor 10x DIC L N1 dry 20X 0.75 NA
• Plan Apo VC 20x DIC N2 dry
• Plan Fluor 40x Oil DIC H N2
• Plan Apo 60x Oil λS DIC N2
• Plan Fluor 100X 1.49 NA, Oil, DIC

Laser Lines(nm):

404, 457, 476, 488, 514, 568, and 638

Filter Cubes(eyes):

DAPI, FITC, TRITC, CFP, YFP, RGB

Accessories:

• Tokai Hit incubation stage chamber at 37ºC, optional 5% CO2
• Motorized x-y stage
• Z-stacks
• Nikon Perfect Focus System

Acquisition Software:

The Nis Elements 5.0 Imaging Software contains a cutting-edge analysis package of modules for doing quantification and densitometry of fluorescence in 2D and 3D.  Nis Elements can also be used to count cells and track cells on time-lapse movies.

We offer high-end workstations computers (Analysis PC 1 ,2, and 3) for image processing and analysis. Our workstation computers allow for image analysis without hindering access to computers operating microscopy equipment.

Analysis PC1:

• Nikon NIS-Elements wish deconvolution and SIM reconstruction modules is optimized for advanced research applications, Nikon’s flagship software package features fully automated image acquisition, advanced device control and powerful analysis and visualization tools. From Single to Multi-Dimensional imaging. Intuitive display of multi-dimensional datasets with multiple viewing options (e.g. slice, 3D volume, time, spectral, binary, etc.), easy data management, and export ready. From simple manual measurements to automatic data collection of hundreds of parameters. Full suite of binary and image processing tools in General Analysis (GA) Multi-channel binary software assay toolbox, 2D and 3D Tracking and Measurement, Realtime analysis of live cell dynamics during acquisition, Intensity Based Measurements, Morphological Based Measurements, Object Tracking, 3D Volume Measurements and counting. Easily view tiled/stitched large images, Unique Extended Depth of Focus (EDF) view for creating beautiful, high-contrast, in-focus 2D projection images from 3D data, Advanced 3D and 2D deconvolution algorithms for extending resolution, Unique visualization options such as XYT view.
• Agilent BioTek Gen5 Imaging & analysis software offers advanced processing, and analysis for a broad range of images generated on LinheartFX and other Agilent-BioTek equipment.

Analysis PC2:

• Zeiss Arivis Pro is modular image analysis software when working with multi-channel 2D, 3D, and 4D images of virtually unlimited size. This software can process large data sets of more than a Terabyte in size. Highly scalable computing, not dependent on local system resources. Processing and quantifying any kind of multidimensional image data. Combining different operators for complex analysis. Denoising, AI-driven segmentation, filtering, thresholding and more.
• Zeiss Zen BlueZEN is the universal user interface you will see on every imaging system from ZEISS. For simple and routine works, ZEN leads you straight to result. For complex research experiments, ZEN offers the flexibility to design multi-dimensional workflows the way you wanted. No matter what microscopy task you have, you will find intuitive tools and modules to assist you:Acquire images using smart automation, Process images with scientifically proven algorithms, visualize big data by GPU powered 3D engine, Analyze images via Machine Learning-based tools, Quantitative Processing. More than 180 image processing tools help you transform and manage your data. ZEN will read the metadata of the input image, then display only the logical processing steps, and optimize the default parameters automatically. You can even process images from other platforms using third-party import tools. With a dedicated workspace, you can also batch process multiple images with ease for quantitative and unbiased results. Quickly generate a huge 2D tile scan with multi-channels at high magnification, and smoothly zoom in and out with a simple mouse scroll.
• Nikon NIS-Elements with denoise function.

Analysis PC3:

• Bitplane Imaris 10.1 with Machine Learning. A state-of-the-art software package designed specifically for quantitative analysis of fluorescence microscopy data. Each image taken on a microscope has a huge array of quantitative information – protein locations, organelle morphology, Imaris is the leading visualization and analysis software for widefield, confocal, light sheet, two-photon, electron microscopy, CLEM, OPT and other imaging modalities. Imaris for Core Facilities provides smart detection and visualization of complex objects, tracing of neurons, blood vessels and other filamentous structures, 3D tracking (including cell division detection), interactive plotting and batch analysis. Additionally, Imaris can handle extremely large (terabytes) data files.