Protein mass spectrometry 2004
(BMG 744)
Course will be held on Tuesdays and Fridays 9 - 11 am in the McCallum Building, 4th floor conference room (401)
Schedule
| Date | Lecturer | Topic |
|---|---|---|
| Jan6 | S Barnes /H Kim | The world of proteins - beyond genomics [PDF File] |
| Jan 9 | H. Kim | The proteome, proteomics and where to start [PDF File] |
| Jan 13 | Liz Brandon | Isolation of specific cells and subcellular fractions [PDF FIle] |
| Jan 16 | M Baggott | Techniques of protein separation [PDF File] |
| Jan 20 | H Kim | Protein separation by electrophoresis and other 2D-methods |
| Jan 23 | Student presentations | |
| Jan 27 | S Barnes | Mass spectrometry of proteins and peptides: principles and principal methods [PDF File] |
| Jan 30 | S Barnes | MALDI and peptide mass fingerprinting [PDF File] |
| Feb 3 | S Barnes | Interpretation of peptide fragmentation spectra - peptide sequencing and posttranslational modifications [PDF File] |
| Feb 6 | Class demo of methods | |
| Feb 9 | Mid-term exam | |
| Feb 13 | E Lefkowitz | Connecting proteomics into bioinformatics [PDF File] |
| Feb 17 | S Meleth | Statistical issues in proteomics and mass spectrometry [PDF FiIe] |
| Feb 20 | S Barnes | Qualitative and quantitative burrowing of the proteome [PDF File] |
| Feb 24 | H Kim/Townes | Protein-protein networks/Affinity isolation/immunoprecipitation |
| Feb 27 | P Prevelige | Protein structure by H-D exchange mass spectrometry |
| Mar 2 | S Barnes | Enzymology, proteomics and mass spectrometry [PDF File] |
| Mar 5 | Student presentations | |
| Mar 9 | S Barnes / Wang | Tissue and fluid proteomics [PDF File] |
| Mar 12 | H Kim | Application of proteomics to the brain proteome |
| Mar 16 | V Darley-Usmar | The mitochondrial proteome |
| Mar 19 | Final exam |