Protein mass spectrometry 2010
(BMG/PHR 744)
The science of proteomics and mass spectrometry
Course will be held on Tuesdays and Fridays 9 - 11 am in the McCallum Building, 4th floor conference room (401)
Schedule
| Date | Lecturer | Topic |
|---|---|---|
| Jan 5, Tu | S. Barnes/H. Kim | The world of biomolecules. The proteome, proteomics and other –omics and where to start [PDF File] [Questions] |
| Jan 8, Fri | M. Renfrow | Mass spectrometry – gas phase transfer and instrumentation – include ETD [PDF File] [Questions] |
| Jan 12, Tu | S. Barnes | Methods for the identification of proteins: MALDI-TOF of proteins and peptide mass fingerprinting; LC analysis and peptide sequencing [PDF File] [Questions] |
| Jan 15, Fri | S. Barnes | Ion
fragmentation in mass spectrometry; application to proteomics
[PDF
File] [Questions] |
| Jan 19, Tu | S. Barnes | Isotopes in mass spectrometry [PDF File] [Questions] |
| Jan 22, Fri | J. Prasain | Ion fragmentation of small molecules; Lipidomics [PDF File] [Questions] |
| Jan 26, Tu | S. Barnes/ S. Asmellash |
Sample preparation for proteomics and mass spectrometry [PDF File] |
| Jan 29, Fri | E. Shonsey | MS in Forensics [PDF File] |
| Mid-term exam – take-home | ||
| Feb 2, Tu | H. Kim | Simplifying the proteome - techniques of protein purification; protein separation by electrophoresis and other 2D-methods [PDF File] [Questions] |
| Feb 5, Fri | A. Smith IV | Connecting proteomics into bioinformatics; MUDPIT and SEQUEST; false discovery rates in complex systems [PDF File] |
| Feb 9, Tu | C. Crasto | The bioinformatics of the proteome [PDF File] |
| Feb 12, Tu | S. Barnes | Mass spectrometry in qualitative and quantitative burrowing of the proteome [PDF File] |
| Feb 16, Fri | J. Prasain | Designing the proteomics and metabolomics experiment [PDF File] [Questions] | Feb 19, Fri | J. Prasain | Qualitative and quantitative analysis/method validation in metabolomics [PDF File] [Questions] |
| Feb 23, Tu | S. Barnes | Enzymology and mass spectrometry [PDF File] |
| Feb 26, Fri | M. Renfrow | Analysis of protein-protein interactions by affinity purification and
mass spectrometry [PDF
File 1] [PDF
File 2] [PDF
File 3] [Bring a copy
of the supplemental info (not the whole thing, just the methods)] |
| Mar 2, Tu | P. Prevelige | Mass spectrometry as a tool for studying protein structure |
| Mar 5, Fri | P. Prevelige | Study of macromolecular structures - protein complexes |
| Mar 9, Tu | M. Renfrow | Applications of FT-ICR-MS |
| Mar 12, Fri | J. Novak/ M. Renfrow |
Mass spectrometry in glycomics research - application to IgA nephropathy [PDF File] |
| Mar 16, Tu | C-C. Wang | Tissue and body fluid proteomics and mass spectrometry |
| Mar 19, Fri | J. Mobley/ D. Stella |
Applications of MS to tissue imaging |
| Mar 23, Tu | H. Kim | Use of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases [PDF File] |
| Final report due |