Protein mass spectrometry 2012
(BMG/PHR 744)
The science of proteomics and mass spectrometry
Course will be held on Mondays, Wednesdays and Fridays 9 – 10:30 am in the McCallum Building, 4th floor conference room (401)
Schedule
| Date | Lecturer | Topic |
|---|---|---|
| Basics Course | ||
| Jan 6, Fri | S. Barnes/H. Kim | Analyzing biomolecules. The proteome, proteomics and other –omics and where to start [PDF File] |
| Jan 9, Mon | M. Renfrow | Mass spectrometry – gas phase transfer and instrumentation – include ETD [PDF
File] Youtube Movies[Quadrupole, FT-ICR MS, Triple Quad, Synapt G2 (ion mobility)] |
| Jan 11, Wed | M. Renfrow | Methods for the identification of proteins: MALDI-TOF of proteins and peptide mass fingerprinting; LC analysis and peptide sequencing [PDF File] |
| Jan 13, Fri | M. Renfrow | Ion fragmentation in mass spectrometry; application to proteomics [PDF File] |
| Jan 16, Mon | TBA | Sample preparation for proteomics and mass spectrometry |
| Jan 18, Wed | H. Kim | Simplifying the proteome - techniques of protein purification [PDF File] | Jan 20, Fri | H. Kim | Protein separation by electrophoresis and other 2D-methods [PDF File] |
| Jan 23, Mon | S. Barnes | Qualitative burrowing of the proteome [PDF File] | Jan 25, Wed | S. Barnes | Quantitative burrowing of the proteome [PDF File] |
| Jan 27, Fri | TBA | MUDPIT and SEQUEST; false discovery rates in complex systems |
| Jan 30, Mon | S. Barnes | MRMPath; MRMutation; MRMass Space [PDF File] |
| Feb 1, Wed | C. Crasto | Web tools and the proteome; Expasy, KEGG, NCBI, others (Location: Shelby 105 at 8 am) [PDF File (14MB)] | Feb 3, Fri | J. Prasain | Lipidomics and other small molecules by LC-MS [PDF File] | Feb 6, Mon | Exam |
| Applications Course | ||
| Feb 8, Wed | J. Prasain | Metabolomics – LC-MS and GC-MS [PDF File] |
| Feb 10, Fri | J. Prasain | Quantitative analysis/method validation in metabolomics [PDF File] |
| Feb 13, Mon | S. Barnes | Enzymology and mass spectrometry [PDF File] |
| Feb 15, Wed | Student presentations | |
| Feb 17, Fri | M. Renfrow | Analysis of protein-protein interactions by affinity purification and mass spectrometry [PDF File 1] [PDF File 2] [PDF File 3] |
| Feb 20, Mon | P. Prevelige | Mass Spectrometry as a Tool for Studying Protein Structure |
| Feb 22, Wed | P. Prevelige | Study of macromolecular structures – protein complexes [PDF File 2] | Feb 24, Fri | E. Shonsey | MS in Forensics [PDF File 2] |
| Feb 27, Mon | TBA | Tissue and body fluid proteomics and mass spectrometry |
| Feb 29, Wed | Student presentations | |
| Mar 2, Fri | D.Stella/ S. Barnes |
Applications of MS to tissue imaging – the lens [PDF File] |
| Mar 5, Mon | M. Renfrow | Applications of FT-ICR-MS |
| Mar 7, Wed | J. Novak/ M. Renfrow |
Mass spectrometry in glycomics research - Application to IgA nephropathy |
| Mar 9, Fri | H. Kim | Use of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases [PDF File] |
| Mar 12, Mon | H. Kim/S. Barnes | Putting it all together – by-passing pyruvate kinase |
| Mar 14, Wed | S. Barnes | Isotopes in mass spectrometry [PDF File] |
| Mar 16, Fri | S. Barnes | Applying mass spectrometry to Free Radical Biology |
| Mar 19, Mon | Final report due |