Explore UAB

The BIOEMTECH β-Eye PET and BIOMTECH γ-Eye SPECT imaging systems enable 2D PET and SPECT imaging of small animals infected with high containment pathogens. These systems enable SEBLAB investigators to leverage the use of novel radionuclide:antibody conjugates generated by UAB’s TR24 cyclotron (link). Performance of PET and SPECT on infected live animals enables highly sensitive, non-invasive dynamic imaging of the entire body of the same animal over time, providing novel opportunities to develop non-invasive diagnostic imaging tests for difficult to diagnose infections.

β-eye - Bioemtech | γ-eye - Bioemtech

The MiLabs µCT^UHROI^FLT In Vivo Imaging System enables 3D imaging of infected small and medium-sized animals utilizing ultra-high resolution computed tomography (CT) coupled with fluorescent, bioluminescent, and Cherenkov optical imaging. This capability enables longitudinal repeat imaging of infection-mediated tissue pathology in mice, rats, ferrets, and rabbits without the need to sacrifice the animal, providing more detailed and dynamic insights into tissue-level pathogenesis with optimized efficiency, enhanced reproducibility, and decreased animal use.

The ZEISS Lattice Lightsheet 7 is a powerful microscope designed for long-term volumetric imaging of living cells at subcellular resolution. It utilizes a unique lattice light sheet illumination technique to minimize photobleaching and phototoxicity, allowing you to observe delicate biological processes over extended periods without harming samples.

The Cytation 10 confocal imaging reader combines the functionalities of a multi-mode microplate reader with automated confocal and widefield microscopy. This unique design makes it a versatile tool for researchers working in various fields like cell biology, drug discovery, and protein analysis.

The CosMx Spatial Molecular Imager (SMI) is a powerful tool used in the field of spatial biology. It is a high-plex, single-cell imaging platform that allows researchers to analyze gene expression and protein abundance within tissues at a cellular and subcellular level. This technology provides valuable insights into various biological processes, such as cell-cell interactions, tissue development, and disease progression.

The Visium CytAssist is a compact instrument designed to transfer transcriptomic probes from frozen tissues as well as formalin-fixed paraffin embedded (FFPE) tissues on standard glass slides to proprietary Visium slides, enabling genome-wide spatial transcriptomics and detailed insights into host-pathogen interactions during tissue infection.

The Echo Revolve fluorescence microscope is a hybrid microscope that performs brightfield, darkfield, phase contrast, and polarized light microscopy in addition to fluorescence microscopy in an upright configuration for glass slides and inverted setup for live samples in dishes, wells, and flasks. Image acquisition and analysis is also performed on an integrated tablet.

The Buxco Inhalation Exposure system enables the simultaneous and precise delivery of microbes, toxins, small molecules, biologics, and more into the lungs of anesthetized mice and rats.

Glas-Col Inhalation Exposure System is a whole-body exposure chamber that enables controlled and reproducible exposure of small animals to airborne pathogens or contaminants. It utilizes a nebulizer-venturi unit to aerosolize the pathogenic suspension, with adjustable parameters for concentration, air flow rate, and exposure duration. 

The FlexiVent Pulmonary Function Testing system is widely regarded as the gold standard for the measurement of respiratory mechanics in live animals. This system utilizes a method called forced oscillation technique (FOT) to deliver a small oscillating signal to the airways and measure the resulting pressure, flow, and volume changes.

The MACSQuant Tyto is a next generation, benchtop cell sorter equipped with 3 lasers, which allows for 10-parameter cell sorting. Samples are loaded onto a proprietary single-use, disposable, and fully closed cartridge with microfluidic technology enabling gentle cell sorting.

The Agilent Seahorse XF Pro Analyzer measures the oxygen consumption rate and extracellular acidification rate of live cells in a 96-well format. Immuno-metabolism is a burgeoning field given its significant role in mediating innate and adaptive immune cell activation, proliferation, and memory responses to infectious agents during active and latent infection. Excess inflammation is a hallmark of severe disease caused by RGIII pathogens including RNA viruses with pandemic potential and this technology enables unique investigation into the immune metabolic fluxes mediating this process.

The Applied Biosystems QuantStudio 7 Dx Pro is a FDA-cleared RT-PCR system for In Vitro Diagnostic (IVD) use in clinical labs. With a compact design, very high sensitivity, broad linear dynamic range, and the ability to support up to six-color multiplexing, this system is ideal to support the development of novel multiplexed molecular diagnostic assays and quantify viral loads, organism burden, and gene expression in research samples.

The Applied Biosystems ProFlex PCR system is an industry leading thermocycler with three distinct bays capable of simultaneously running three different PCR conditions and changing between 5 different blocks with the flip of a switch.

The Maxwell® RSC 48 Instrument is a compact, automated nucleic acid purification platform that processes up to 48 samples simultaneously. Using Maxwell® RSC prefilled cartridges, the Maxwell® RSC 48 Instrument brings the same consistent, reliable purification of DNA or RNA from a variety of sample types and a higher throughput. The intuitive graphical interface makes the instrument easy to use. An integrated Bar Code reader makes it easy to track samples.

The QIAcuity Digital PCR System enables precise, multiplexed, and absolute quantification of PCR amplification targets. Applications include absolute quantification of gene targets of interest and pathogens (e.g. viral load) without the need for standard curves. This nanoplate-based system seamlessly integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time.

The Chromium X Single Cell platform was designed to help make single cell studies accessible, approachable, and easy for everyone. Versatile assays are part of end-to-end workflows that combine with user-friendly instruments for efficient cell partitioning and barcoding, plus intuitive software for data analysis. Chromium X Series provides robust, reliable single cell assays including gene expression, immune profiling, chromatin accessibility, CRISPR screening, and cell surface protein expression.

Ella is a next-generation benchtop automated ELISA platform designed to deliver accurate, reproducible data with no manual steps. It enables the development of novel multi-target assays that are easy to perform with minimal hands on time with sub-picogram sensitivity in ~90 minutes, enabling real-world translation into less complex clinical and public health labs. This system offers customizable cartridges for novel assay development and a wide range of validated cytokine arrays pertinent to all SEBLAB users.

The TissueLyser III is well-suited for high-throughput disruption of animal and plant tissues, bacteria and yeast. Highly reproducible purification of high-quality DNA and RNA is achieved, even with difficult-to-lyse samples. The TissueLyser III simultaneously disrupts multiple biological samples through high-speed shaking in tubes or 96-well plates with stainless steel, zirconium, ceramic or glass beads. Using the appropriate adapter set, up to 192 samples can be processed at the same time. Alternatively, a grinding jar set can be used to process large samples.