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2006 UAB Botanicals Workshop
Purdue University and the University of Alabama at Birmingham Botanicals Center for Age-Related Disease
September 11-12, 2006
263 Bevill Research Building
University of Alabama at Birmingham
Birmingham, AL 35294
Workshop Schedule
| Monday, September 11 | |
| 8:00 - 8:30am | Breakfast |
| 8:30 - 8:45am | Introduction to Workshop - S. Barnes/H. Kim/JM. Wyss |
| 8:45 - 9:15am | Selection of animal models - D. Waters (Purdue) - Animals: knockout, transgenic; Cells (mechanism) |
| 9:15 - 10:00 am | Collecting biological samples - H. Kim, S.
Wang, JM. Wyss - metabolic cages, blood sampling, interstitial fluids/aqueous humor, frozen tissues, subcellular fractionation |
| 10:00 - 10:20 am | Coffee break |
| 10:20 - 11:00 am | Selection of botanicals - S. Barnes - previous use by other investigators, defined product, safety data, dose selection, existing pharmacokinetic data, dealing with the supplier/manufacturer, mixing botanicals in the diet, sampling the diet |
| 11:00 - 11:30 am | Analysis of the botanical - J. Prasain - extraction and LC methods (UV diode, electrochemical) |
| 11:30 - 12 noon | LC-MS methods - S. Barnes, J. Prasain - Ionization methods (ESI, HN-APCI), Qualitative analysis (MS, MS-MS), Quantitative analysis (SIM and MRM) |
| 12:00 - 1:00 pm | Lunch |
| 1:00 - 1:40 pm | Characterizing the animal model - JM. Wyss - blood pressure, maze, cataracts |
| 1:40 - 2:20 pm | Physical methods in models of cataract disease - O. Srivastava |
| 2:20 - 3:00 pm | Studying botanicals and skin models of disease |
| 3:00 - 3:20 pm | Coffee break |
| 3:20 - 5:00 pm | The Botanical Center Facilities |
| Tuesday, September 12 | |
| 8:00 - 8:30 am | Breakfast |
| 8:30 - 8:35 am | Introductions (for proteomics workshop guests) |
| 8:35 - 8:55 am | Biology: the basis for smart proteomic approaches to protein analysis - H. Kim - Choice of organ or cells, whole cell lysate, subcellular fractionation, tagged complexes |
| 8:55 - 9:15 am | Proteomics and Thiol Modification - A. Landar - 2D-gels (IEF/SDS, blue native) |
| 9:15 - 9:35 am | Protein purification prior to proteomics analysis - S. Barnes - Chromatofocusing, Anion/cation exchange, Hydrophobic, Gel filtration |
| 9:35 - 9:55 am | Detecting and quantifying proteins on gels - H. Kim - Staining methods (Coomassie blue, Sypro ruby, Pro-Q diamond, etc.) - Cy-Dye labeling |
| 9:55- 10:15 am | Statistical Experimental Design in Proteomics - S. Meleth - DeCyder |
| 10:15 - 10:40 am | Coffee break |
| 10:40 - 11:10 am | Phosphorylationand Glycosylationby ECD - M. Renfrow - MALDI, electrospray ionization - Detectors (TOF and TOF/TOF, Quadrupole, FT-ICR, Orbitrap, Hybrid detectors) - mass accuracy and mass resolution |
| 11:10 - 11:40 am | Qualitative Proteomics - J. Mobley - peptide mass fingerprinting (MASCOT), peptide MUDPIT (2D-SAX-rpLC-tandem mass spectrometry) (SEQUEST) |
| 11:40 - 12:10 pm | Quantification in the world of proteomics - S. Barnes - peptide coverage, iCAT labeling, iTRAP labeling, 180/160 labeling |
| 12:15 - 1:15 pm | Lunch |
| 1:15 - 2:15 pm | Detection of post-translational modifications of peptides - S.
Barnes Post-translational Modifications to Human Bile Acid CoA:Amino Acid Nacyltransferase - E. Shonsey Localization of 4HNE modifications - S. Eliuk |
| 2:15 - 3:00 pm | Profiling and Imaging Mass Spectrometry - J. Mobley |
| 3:00 - 3:20 pm | Coffee break |
| 3:20 - 5:00 pm | Demonstrations of proteomics and mass spectrometry applications at UAB |