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Sample: human hair, finger/toenails (human). Non-invasive and can be collected, stored and shipped to the BARB Core at room temperature, using regular mail, without any special biohazard requirements or costs.
Equipment: Qiagen TissueLyser III, Biotek Synergy 2 Multi-mode microplate reader
Publications: dietary inflammation, discrimination and stress, chronic cortisol in adult cancer survivors, gardening intervention trial in cancer survivors.
Sample: frozen serum/plasma (human, mouse)
Equipment: Applied Biosystems StepOnePlus Real-Time PCR System, Applied Biosystems DynaMag Magnet, Biotek Synergy 2 Multi-Mode Microplate Reader, LabNet Plate Spinner
Publications: HIV and Chronic Pain
- Complex I (NADH:Ubiquinone Oxidoreductase) Assay
- Complex II (Succinate-Ubiquinone Oxidoreductase) Assay
- Complex III (Decylubiquinol Cytochrome C oxidoreductase) Assay
- Complex IV (Cytochrome c oxidase) Assay
- Complex V (ATP hydrolysis) Assay
- Citrate Synthase Assay
Sample: fresh or frozen tissues or cells (human, rodent, fish, Drosophila)
Equipment: Beckman DU 800 Spectrophotometer, Custom-Built Mitochondrial Isolation Stations with Recirculating Chiller
Training: Please contact us to schedule
Publications: skeletal muscle in rheumatoid arthritis, TrxR2 and glucose tolerance, neonatal hippocampus development, brain IGF-1 and aging, mitochondrial fusion and exercise
- High Resolution Respirometry (HRR) - Oxidative Phosphorylation ONLY
Sample: fresh, permeabilized, ex vivo tissues (human muscle, rodent renal cortex, medulla, and microvessels, embryonic and adult heart, gastroc and soleus muscle, hippocampus, bladder, liver) or isolated mitochondria (rodent, fish, Drosophila)
Equipment: 3 x Oroboros Oxygraph-2k Fluorometers (LED2-Module Amperometric Add-On)
Training: Please contact us to schedule
Publications: neonatal lung injury, skeletal muscle in rheumatoid arthritis, embryonic heart cardiogenesis, soleus muscle in spinal cord injury, post-exercise skeletal muscle, neonatal hippocampus development, aerobic exercise training
- Seahorse Extracellular Flux Analysis (XFe96) – Oxidative Phosphorylation and Glycolysis
Sample: cultured cells or freshly isolated mitochondria (human, rodent), mycoplasmas.
Equipment: Agilent Seahorse XFe96, high throughput, 96-well format
Training: Please contact us to schedule
Publications: neonatal lung injury, ovarian cancer, breast cancer, bronchopulmonary dysplasia, adrenal neuroendocrine cancer, glucose tolerance, mammary tumor-associated macrophages, diabetic retinopathy
- For High Resolution Respirometry (HRR)
- For Mitochondrial Electron Transport Chain (ETC) Kinetics
- Catalase
Sample: plasma, serum, total homogenate
- Glutathione Tietze (GSSG and GSH)
Sample: plasma, whole blood
- Glutathione Peroxidase (GPX)
Sample: plasma, total homogenate
- Total Antioxidant Capacity (TAC)
Sample: plasma, serum, urine, saliva
- Ferric Reducing Antioxidant Power (FRAP)
Sample: plasma