Leica Stellaris5 on a DMI8 microscope base and anti-vibration table is an advanced point scan confocal microscope equipped with automated x-y-z- stage. The system includes four highly sensitive Power HyD® S detectors combined with Acousto Optical Beam Splitter (AOBS®), 405 solid state laser, and an exclusive White Light Laser that can accommodate any excitation wavelength between 485 nm and 790 nm. The features of Stellaris5 including unique fluorescence lifetime based TauSense® technology allowing for extended detection range (up to 850 nm), up to 8 simultaneous excitation wavelengths for imaging of spectrally overlapping fluorophores, removal of unwanted fluorescence, advanced separation of fluorescent signals, access to functional information of fluorescent specimen (metabolic status, pH, ion concentration), and detection and quantification of protein interactions. In addition, the system can generate super-resolution images at 120nm scale with the adaptive LIGHTNING® detection. Users are able to image with a wide variety of microscopy techniques including phase-contrast, fluorescence, fixed samples, as well as live specimens that require temperature, humidity, and CO2 control. The microscope is equipped with a wide range of objectives; HC PL APO CS2 63x/1.40 OIL, HC PL FLUOTAR 5x/0.15 DRY, HC PL APO CS2 10x/0.40 DRY, HC PL APO CS2 20x/0.75 IMM, HC PL APO CS2 63x/1.40 OIL, 20x/0.70 DRY HCX PL FLUOTAR,1.6x/0.05 DRY. The Stellaris5 microscope is operated with LAS X software and support from HRIF staff and Leica specialists.