Antibody Characterization and Serology (ACS) Core
Goal of the ACS core: The goal of the Antibody Characterization and Serology (ACS) core is to offer research reagents, multiplex immunoassays and high-throughput technologies that help researchers (i) investigate B cell responses to infectious agents and autoimmune diseases, (ii) quantitate antigen-specific antibodies, (iii) measure multiple cytokines and other markers, and (iv) assess binding kinetics/affinity for antibody-antigen interactions and many other molecular interactions.
Services by the ACS core: The four services that will be offered by the ACS core are (i) the supply of viral recombinant antigens (including biotin-labelled antigens) and B-cell tetramers to investigate B cell responses to infectious agents, (ii) the detection and quantitation of antigen-specific antibodies by cytometric bead array (CBA) immunoassays, (iii) multiplex detection and measurement of cytokines and other markers by Luminex® xMAP® technology, and (iv) high-throughput analysis of biomolecular interactions using the Alto surface plasmon resonance system.
Core personnel: Davide Botta (Supervisor) dbotta@uab.edu and Fen Zhou (Specialist) fzhou@uab.edu
Core location: SHEL 571
Instrument used for the detection and quantitation of antigen-specific antibodies by cytometric bead array (CBA) immunoassays: CytoFLEX (Beckman Coulter)
Instruments used for multiplexing detection and measurement of cytokines and other markers: MagPix and LX200 (Luminex)
Instrument used for high-throughput analysis of biomolecular interactions - the Alto surface plasmon resonance system (Nicoya)
Other photos of the ACS core
