Description: We offer (through the two providers listed below), a list of “Metabolic Flux Analysis” workflows, which include longitudinally measured heavy labeled (13C/ 15N) “parent” metabolites as a way of tracking downstream “daughter” metabolites through a specific pathway (ex:13C glucose or BCAA/ Valine as the substrait or parent metabolite) in biological specimens (ex: mouse models and cells in culture) using either relative or absolute quantitative analysis that include a wide array of polar and non-polar down stream product or “daughter” metabolites. This can be hypothesis-driven or utilized as a verification/ validation approach involving a workflow that includes LC-MS2 analysis carried out on extracted metabolites along with internal standards run in either positive or negative modes with high-resolution mass spectrometry (HRMS). The data is analyzed using peak intensities of parent and/ or key daughter ions for absolute or relative quantification computed using either a heavy labeled small molecule of the same or similar chemistries as internal standard curves, in many cases, these metabolites are part of a specific pathway and/ or identified as part of a discovery experiment following validation using a targeted application. These data are then compared across groups using an appropriate statistical analysis. For further reading about targeted and flux-based metabolomics experiments, please see the references below.
- Reference: Federica Danzi, et. Al “To metabolomics and beyond: a technological portfolio to investigate cancer metabolism” Signal Transduction and Targeted Therapy volume 8, Article number: 137 (2023)
- Reference: Silvia Radenkovic, Ivan Vuckovic, & Ian R. Lanza “Metabolic Flux Analysis: Moving beyond Static Metabolomics” Technology of the Month | Volume 45, Issue 6, P545-546, June 2020
Providers: Please Contact Dr. Mobley (
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UAB Analytical Methods in Targeted Metabolomics and Proteomics Laboratory (TMPL)
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- Contacts:
- Director, Stephen Barnes, Ph.D. (
sbarnes@uab.edu ) - Landon Wilson (
empy1977@uab.edu ; PH: (205) 934-3462)
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Web: TMPL
- UAB SOM IRCP & O'Neal CCC Mass Spectrometry/ Metabolomics (MSM) Shared Resource
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- Contacts:
- Director, James Mobley, Ph.D. (
mobleyja@uab.edu ) - Kyoko Kojima, Ph.D. (
kkojima@uab.edu ; PH: (205) 996-6213)
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