Description:   We offer (through the two providers listed below), a list of “targeted” metabolomics workflows to generate either relative or absolute quantitative analysis of any biological specimen for a wide array of polar and non-polar metabolites. This is a generally a verification/ validation approach involving a workflow that utilizes LC-MS2 analysis carried out on extracted metabolites along with internal standards run in either positive or negative modes with high resolution mass spectrometry (HRMS). The data is analyzed using peak intensities of parent and/ or key daughter ions for absolute or relative quantification computed using either a heavy labeled small molecule of the same or similar chemistries as internal standard curves, in many cases these metabolites are part of a specific pathway and/ or identified as part of a discovery experiment. These data are then compared across groups using an appropriate statistical analysis. In some cases, a pathway specific metabolite that is found to be differentially abundance will be further studied using a heavy labeled time-based tracking technique that can also be carried out with a similar relative or absolute quantitative analysis using what is commonly referred to as “Metabolic Flux Analysis”. For further reading about targeted and flux-based metabolomics experiments please see the references below.  

• Reference: Federica Danzi, et. Al “To metabolomics and beyond: a technological portfolio to investigate cancer metabolism” Signal Transduction and Targeted Therapy volume 8, Article number: 137 (2023)
• Reference: Silvia Radenkovic, Ivan Vuckovic, & Ian R. Lanza “Metabolic Flux Analysis: Moving beyond Static Metabolomics” Technology of the Month | Volume 45, Issue 6, P545-546, June 2020

Providers: Please Contact Dr. Mobley (This email address is being protected from spambots. You need JavaScript enabled to view it.) or go to the direct links below to other facilities for more information.

• • • Penn Medicine, UPenn Abramson Cancer Center & Perelman SOM Cardiovascular Institute Metabolomics Core
      • • Contacts:
        • Director: Daniel Kelly, MD (This email address is being protected from spambots. You need JavaScript enabled to view it.)
        • Technical Director/ Director: Christopher Petucci, Ph.D. (This email address is being protected from spambots. You need JavaScript enabled to view it.; PH: (215) 898-9765)

Web: Penn Medicine - Abramson Cancer Center

Web: UPenn - CVI - Metabolomics Core

• • • UAB Analytical Methods in Targeted Metabolomics and Proteomics Laboratory (TMPL)
      • • Contacts:
        • Director: Stephen Barnes, Ph.D. (This email address is being protected from spambots. You need JavaScript enabled to view it.)
        • Landon Wilson (This email address is being protected from spambots. You need JavaScript enabled to view it. PH: (205) 934-3462)

Web: TMPL