Description:   We offer (through the two providers listed below), unbiased relative quantitative analysis of any biological specimen for a wide array of polar and non-polar metabolites. This is a hypothesis driven approach with a workflow that includes the use of LC-MS2 analysis carried out on extracted metabolites run in both positive and negative modes using high resolution mass spectrometry (HRMS). The data is then searched using databases of known metabolites with known masses, retention times, and fragmentation patterns. Once metabolites are matched with high confidence, peak intensities are then measured and normalized across each run, these lists are then compared across groups using the appropriate statistical analysis, which is then followed by systems/ pathway analysis. In many cases, once a handful of differential metabolites are found to be of interest, verifications/ validations can be carried out with a relative or absolute quantitative analysis using a specific and fine-tuned “Targeted Small Molecule/ Metabolite” LC-MS2 approach. For further reading please see the reference below.  

• Reference: Federica Danzi, et. Al “To metabolomics and beyond: a technological portfolio to investigate cancer metabolism” Signal Transduction and Targeted Therapy volume 8, Article number: 137 (2023)

Providers: Please Contact Dr. Mobley (This email address is being protected from spambots. You need JavaScript enabled to view it.) or go to the direct linksbelow to other facilities for more information.

• Penn Medicine, UPenn Abramson Cancer Center & Perelman SOM Cardiovascular Institute Metabolomics Core
  • • • Contacts:
      • Director: Daniel Kelly, MD (This email address is being protected from spambots. You need JavaScript enabled to view it.)
      • Technical Director/ Director: Christopher Petucci, Ph.D. (This email address is being protected from spambots. You need JavaScript enabled to view it.; PH: (215) 898-9765)

Web: Penn Medicine - Abramson Cancer Center

Web: UPenn - CVI - Metabolomics Core

• Metware Biotechnology Inc.; Untargeted, Targeted and Widely Targeted Metabolomics
  • • Contact:
    • Bobby Wei (This email address is being protected from spambots. You need JavaScript enabled to view it.; PH: (929) 599-0550)

Web: Metware Bio