Indiana O'Brien Center for Advanced Microscopic Analysis
Summary
The Indiana University George M. O’Brien Center for Advanced Microscopic Analysis has a unique, highly interactive, integrated and synergistic team of physicians, basic scientists, computer scientists, engineers and a highly trained and committed technical support team to provide local, national and international users cutting edge intravital optical microscopy and 3-dimensional quantitative digital image analysis of the kidney. They have two cores (Molecular Imaging and Intravital Microscopy) that offer a novel set of research methods, quantitative analysis tools and fluorescent biosensors or probes not available elsewhere to facilitate biomedical research, drug discovery and therapeutic approaches to kidney disease.
Molecular Imaging Resource Core
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Description
The Molecular Imaging Resource Core provides quantitative, large-scale microscopy of the kidney as a service to kidney and urologic investigators. Services include:
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Services
- 3-dimensional confocal tissue cytometry of kidney tissue
- CODEX multiplexed cytometry of mouse and human kidney tissue
- Spatial Transcriptomics of mouse and human kidney tissue (Visium)
- In situ sequencing of mouse and human kidney tissue
Intravital Microscopy Resource Core
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Description
The Intravital Microscopy Resource Core provides quantitative intravital microscopy of rodent animal models customized to the specific needs of individual kidney and urologic investigators. Assays and techniques include:
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Services
- Glomerular Permeability
- Proximal tubule endocytosis
- Microvascular flow
- Microvascular permeability
- Mitochondrial function
- Cellular oxidative stress
- Apoptosis and necrosis
- Characterization of in vivo fluorescent probe delivery to the kidney
- Characterization of transgenic rodents expressing fluorescently tagged proteins in the kidney
- Fluorescence lifetime imaging of endogenous and exogenous fluorophores in the kidney
Contact Information
If you would like more information regarding services provided by Indiana O’Brien Center for Advanced Microscopic Analysis, please contact:
Joshua Kuhn
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