Indiana O'Brien Center for Advanced Microscopic Analysis
Summary
The Indiana University George M. O’Brien Center for Advanced Microscopic Analysis has a unique, highly interactive, integrated and synergistic team of physicians, basic scientists, computer scientists, engineers and a highly trained and committed technical support team to provide local, national and international users cutting edge intravital optical microscopy and 3-dimensional quantitative digital image analysis of the kidney. They have two cores (Molecular Imaging and Intravital Microscopy) that offer a novel set of research methods, quantitative analysis tools and fluorescent biosensors or probes not available elsewhere to facilitate biomedical research, drug discovery and therapeutic approaches to kidney disease.
Molecular Imaging Resource Core
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Description
The Molecular Imaging Resource Core provides quantitative, large-scale microscopy of the kidney as a service to kidney and urologic investigators. Services include:
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Services
- 3-dimensional confocal tissue cytometry of kidney tissue
- AKI study design and planning for imaging studies
- Animal/material transfer
- CKD study design and planning for imaging studies
- CODEX multiplexed cytometry of mouse and human kidney tissue, other tissues/organs
- Data analysis and visualization
- Data cleaning, filtering and mapping
- Data transfer
- In situ sequencing of mouse and human kidney tissue (Xenium)
- Lightsheet microscopy
- Manuscript development/publication support
- Methods development in microscopy and molecular imaging
- Project consultation for imaging studies
- Rodent cisplatin kidney injury
- Rodent Kidney Ischemia reperfusion injury
- Rodent Nephrectomy
- Rodent sepsis models
- Spatial Transcriptomics of mouse and human kidney tissue (Visium SD, Visium FFPE)
- Training in Digital Image Analysis
Intravital Microscopy Resource Core
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Description
The Intravital Microscopy Resource Core provides quantitative intravital microscopy of rodent animal models customized to the specific needs of individual kidney and urologic investigators. Assays and techniques include:
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Services
- AKI study design and planning for imaging studies
- Animal/material transfer
- Characterization of transgenic rodents expressing fluorescently tagged proteins in the kidney
- CKD study design and planning for imaging studies
- Data analysis and visualization
- Data transfer
- Fluorescence lifetime imaging of endogenous and exogenous fluorophores in the kidney
- Intravital Imaging: Apoptosis and necrosis
- Intravital Imaging: Cellular oxidative stress
- Intravital Imaging: Characterization of in vivo fluorescent probe delivery to the kidney
- Intravital Imaging: Glomerular Permeability
- Intravital Imaging: Microvascular flow
- Intravital Imaging: Microvascular permeability
- Intravital Imaging: Mitochondrial function
- Intravital Imaging: Proximal tubule endocytosis
- Manuscript development/publication support
- Methods development in microscopy and molecular imaging
- Project consultation for imaging studies
- Rodent cisplatin kidney injury
- Rodent Kidney Ischemia reperfusion injury
- Rodent Nephrectomy
- Rodent sepsis models
- Training in Digital Image Analysis
- Training in Intravital microscopy
Contact Information
If you would like more information regarding services provided by Indiana O’Brien Center for Advanced Microscopic Analysis, please contact:
Joshua Kuhn
Website