The George M. O’Brien Kidney Resource Alliance (OKRA) provides over 200 specialized resources, tools, technologies, services, and expertise beyond those typically available in an individual lab or institutional core. If you are interested in requesting any of the resources below, please click the “Request Here” button below. If you have any questions, email us at
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Animal Models/Procedures
Rodent cisplatin kidney injury Indiana University Intravital core/Molecular Imaging Resource cores Rodent Kidney Ischemia reperfusion injury Indiana University Intravital core/Molecular Imaging Resource cores Rodent Nephrectomy Indiana University Intravital core/Molecular Imaging Resource cores Rodent sepsis models Indiana University Intravital core/Molecular Imaging Resource cores Comprehensive mouse kidney phenotyping for diet and stress mechanisms Johns Hopkins Biomedical Resource Core (B-DSSL) Germ-free facility consultation use Johns Hopkins Biomedical Resource Core (B-DSSL) Germ-free sample banks Johns Hopkins Biomedical Resource Core (B-DSSL) GFR measurements Johns Hopkins Biomedical Resource Core (B-DSSL) Humanized mouse diet protocols Johns Hopkins Biomedical Resource Core (B-DSSL) Immunological manifestations of dietary and social stressors in the kidney Johns Hopkins Biomedical Resource Core (B-DSSL) Isolated Nephron segment Ex Vivo Analysis Johns Hopkins Biomedical Resource Core (B-DSSL) Kidney Slice Preparation Johns Hopkins Biomedical Resource Core (B-DSSL) Metabolic Cage and Clearance Studies Johns Hopkins Biomedical Resource Core (B-DSSL) Metabolism phenotyping Johns Hopkins Biomedical Resource Core (B-DSSL) Mouse biobank of dietary and social stress models Johns Hopkins Biomedical Resource Core (B-DSSL) Optical Clearing Johns Hopkins Biomedical Resource Core (B-DSSL) Plasma and Urine Electrolyte Panels Johns Hopkins Biomedical Resource Core (B-DSSL) Protocols, training, and service to isolate and characterize kidney immune cells by flow cytometric analysis (FACS) Johns Hopkins Biomedical Resource Core (B-DSSL) Small Sample RNAseq in Isolated Nephron Segments Johns Hopkins Biomedical Resource Core (B-DSSL) Telemetric Blood pressure Monitoring Johns Hopkins Biomedical Resource Core (B-DSSL) Model systems studies to identify pathways shared cross-species Michigan Applied Systems Biology Core Animal Handling and Husbandry Pittsburgh Model Systems Core Animal Models - Blood pressure telemetry Pittsburgh Model Systems Core Animal Models - Mitochondrial / Bioenergetics (Seahorse) Pittsburgh Model Systems Core Animal Models - Purine metabolomics Pittsburgh Model Systems Core Animal Models - Injury biomarker assessment Pittsburgh Model Systems Core Animal Models - Measurement of GFR and RBF Pittsburgh Model Systems Core Animal Models - Metabolic cage studies, urine metabolites Pittsburgh Model Systems Core Animal Models - Real-time super-resolution ultrasound of kidney microvasculature Pittsburgh Model Systems Core Animal Models - Tissue Harvesting Pittsburgh Model Systems Core Disease Models: AKI (cecal ligation and puncture) Pittsburgh Model Systems Core Disease Models: AKI (cisplatin nephrotoxicity) Pittsburgh Model Systems Core Disease Models: AKI (gentamicin nephrotoxicity) Pittsburgh Model Systems Core Disease Models: AKI (ischemia-reperfusion injury) Pittsburgh Model Systems Core Disease Models: AKI (sickle cell crisis) Pittsburgh Model Systems Core Disease Models: CKD (aristolochic acid) Pittsburgh Model Systems Core Disease Models: CKD (chronic angiotensin II infusion) Pittsburgh Model Systems Core Disease Models: CKD (FSGS) Pittsburgh Model Systems Core Disease Models: CKD (genetic models) Pittsburgh Model Systems Core Disease Models: CKD (two kidney one clip hypertension) Pittsburgh Model Systems Core Disease Models: CKD (unilateral ureteral obstruction) Pittsburgh Model Systems Core Epithelial Models - Mammalian cell culture models (proximal tubule, collecting duct, polarized epithelia) Pittsburgh Model Systems Core Epithelial Models - Organoid models to study kidney injury Pittsburgh Model Systems Core Epithelial Models - Organoid models to study kidney physiology Pittsburgh Model Systems Core Epithelial Models - Organoid models to study nephron development Pittsburgh Model Systems Core Special Rodent Diets (low and high Na+ and K+) Pittsburgh Model Systems Core Yeast Models - Positive genetic selection assays Pittsburgh Model Systems Core Yeast Models - Protein quality control (degradation, ubiquitylation) assays Pittsburgh Model Systems Core Yeast Models - Bioinformatic analysis of genotype-phenotype correlations (TopMed, Clinvar, UK BioBank) Pittsburgh Model Systems Core Yeast Models - Computational identification of functional variants Pittsburgh Model Systems Core Yeast Models - Protein trafficking assays (immunofluorescence, density centrifugation) Pittsburgh Model Systems Core Whole kidney clearance/oxygen consumption studies UAB-UCSD Pre-Clinical Core 5/6th nephrectomy (both steps) UAB-UCSD Pre-Clinical Core Automated tail-cuff blood pressure determination (each, 6 days) UAB-UCSD Pre-Clinical Core Cannulation (indwelling with injection port) UAB-UCSD Pre-Clinical Core Determination of GFR in awake rodents UAB-UCSD Pre-Clinical Core GFR (transcutaneous FITC-sinistrin) in rodents UAB-UCSD Pre-Clinical Core Kidney ischemia/reperfusion (IRI) UAB-UCSD Pre-Clinical Core Laser Capture Microdissection from frozen tissue (~70 pieces individually collected per sample; sample quality and amount appropriate for RNA-Seq or potentially proteomics) UAB-UCSD Pre-Clinical Core Metabolic cage experiments in mice (per animal and week) UAB-UCSD Pre-Clinical Core Micropuncture for nephron function and TG feedback UAB-UCSD Pre-Clinical Core Misc. surgical services involving body wall penetration (per hour) UAB-UCSD Pre-Clinical Core Orthotopic kidney transplantation UAB-UCSD Pre-Clinical Core Radio-telemetry determination of blood pressure UAB-UCSD Pre-Clinical Core Tail vein injection or venipuncture (per animal) UAB-UCSD Pre-Clinical Core Tissue oxygen partial pressure determination in kidney UAB-UCSD Pre-Clinical Core Unilateral Ureteral Obstruction UAB-UCSD Pre-Clinical Core Analysis of VUS in non-COL4A genes through generation and analysis of gene edited models WUSTL Variant Validation Core COL4A3/A4/A5 VUS analysis in vivo by mouse gene editing WUSTL Variant Validation Core Generation of primary cells for Seahorse or stable isotope studies WUSTL Metabolism Core Stable isotope tracing studies in vivo: 15N-glutamine: data provisioning WUSTL Metabolism Core Stable isotope tracing studies in vivo: branched chain amino acids: data provisioning WUSTL Metabolism Core -
Biostatistics/Bioinformatics
Data analysis and visualization Indiana University Intravital core/Molecular Imaging Resource cores Data cleaning, filtering and mapping Indiana University Molecular Imaging Resource core Bioinformatic Analysis Johns Hopkins Biomedical Resource Core (B-DSSL) Biostatistical support (including clinical trials, epidemiological cohorts, case-control studies, survey data, and -omics studies) Johns Hopkins Biomedical Resource Core (C-DSSL) Multi-scalar data analysis and integration Michigan Applied Systems Biology Core Quantitative morphometric analyses of kidney biopsy samples Michigan Applied Systems Biology Core CELLxGENE and Vitessce - access/training/support Michigan Data Analytic Services Core Develop generalized workflow for integrative web-based analysis Michigan Data Analytic Services Core Investigating biomarker association (gene, protein, metabolite level) in clinical existing cohorts through TranSMART instance Michigan Data Analytic Services Core Nephroseq - access/training/support Michigan Data Analytic Services Core TranSMART - access/training/support Michigan Data Analytic Services Core Data analysis - cellular and whole animal physiological studies, or imaging studies Pittsburgh All Cores AKI Study Analysis and Interpretation UAB-UCSD Clinical Core Analyses of study results UAB-UCSD Clinical Core Analysis of pilot data UAB-UCSD Clinical Core Sample size and/or power analysis UAB-UCSD Clinical Core Bioinformatics and biostatistical support WUSTL Metabolism Core Stable isotope flux studies- proximal tubules: 15N-glutamine: data provisioning WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-glucose: data provisioning WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-glutamine: data provisioning WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-palmitate and 13C-oleate: data provisioning WUSTL Metabolism Core -
Data Provisioning
Dietary assessment services to investigate dietary patterns and biomarkers of dietary intake and food metabolism Johns Hopkins Biomedical Resource Core (C-DSSL) Genomics dataset w/ deep phenotyping Johns Hopkins Biomedical Resource Core (C-DSSL) Kidney Precision Medicine Center of Excellence (KPMCOE)—EMR registry Johns Hopkins Biomedical Resource Core (C-DSSL) Large longitudinal cohort study data from racially and socioeconomically diverse populations Johns Hopkins Biomedical Resource Core (C-DSSL) Metabolomics dataset w/ deep phenotyping Johns Hopkins Biomedical Resource Core (C-DSSL) Proteomics dataset w/ deep phenotyping Johns Hopkins Biomedical Resource Core (C-DSSL) Publicly accessible biospecimen repositories and datasets to support or conduct biomarker studies and secondary data analyses Johns Hopkins Biomedical Resource Core (C-DSSL) Clinical and molecular interactions in investigators’ data sets Michigan Applied Systems Biology Core Data storage - imaging files Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Big data transformation & pipeline development UAB-UCSD Clinical Core Cloud-based multimodal databases for AKI and critical care nephrology research studies UAB-UCSD Clinical Core Database development UAB-UCSD Clinical Core Stable isotope flux studies- proximal tubules: 15N-glutamine: data provisioning WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-glucose: data provisioning WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-glutamine: data provisioning WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-palmitate and 13C-oleate: data provisioning WUSTL Metabolism Core -
Laboratory Services
Microbiome Johns Hopkins Biomedical Resource Core (B-DSSL) Biomarker measurement - Clinical Chemistry Johns Hopkins Biomedical Resource Core (C-DSSL) Biomarker measurement - Immunoassays Johns Hopkins Biomedical Resource Core (C-DSSL) Biorepository support - Processing aliquoting, short- and long-term sample storage Johns Hopkins Biomedical Resource Core (C-DSSL) Healthy volunteer samples for Reference Range Johns Hopkins Biomedical Resource Core (C-DSSL) Polymer synthesis for making nanoparticles Johns Hopkins Biomedical Resource Core (C-DSSL) Prospective remnant sample collection Johns Hopkins Biomedical Resource Core (C-DSSL) Stored biospecimen samples from completed studies Johns Hopkins Biomedical Resource Core (C-DSSL) Structured RNA-DNA sequence data integration Michigan Applied Systems Biology Core Fluorophore conjugation with DSPE-PEG NU-USC Biomedical Resource Core In vitro cell viability evaluation - MTS assay/plate reader NU-USC Biomedical Resource Core In Vitro Micelles Binding Evaluation NU-USC Biomedical Resource Core In Vitro Micelles Binding Evaluation: Confocal Microscopy NU-USC Biomedical Resource Core In Vitro Micelles Binding Evaluation: Flow Cytrometry NU-USC Biomedical Resource Core In Vitro Micelles Efficacy Evaluation NU-USC Biomedical Resource Core In Vitro Micelles Efficacy: Quantification of markers related - qPCR NU-USC Biomedical Resource Core In Vitro Micelles Efficacy: Quantification of proteins related - ELISA NU-USC Biomedical Resource Core In Vitro Micelles Efficacy: Quantification of proteins related - Western blot NU-USC Biomedical Resource Core In vitro studies of micelles with Collecting Duct cells - Cortical collecting duct (M1) NU-USC Biomedical Resource Core In vitro studies of micelles with Collecting Duct cells - Cortical collecting duct (Principal cells) NU-USC Biomedical Resource Core In vitro studies of micelles with Collecting Duct cells - Inner Medullary NU-USC Biomedical Resource Core In vitro studies of micelles with Proximal Tubule cells NU-USC Biomedical Resource Core In Vivo Micelles Therapeutic Efficacy: Immunohistochemistry (IHC) Imaging NU-USC Biomedical Resource Core In Vivo Micelles Therapeutic Efficacy: using Rapid Model NU-USC Biomedical Resource Core Incorporation of conjugated siRNA into micelles NU-USC Biomedical Resource Core Micelles formation NU-USC Biomedical Resource Core Nanocarrier/micelles characterization: Morphology - Transmission electron microscopy (TEM) NU-USC Biomedical Resource Core Nanocarrier/micelles characterization: particles size - Dynamic Light Scattering (DLS) NU-USC Biomedical Resource Core Nanocarrier/micelles characterization: Polydispersity Index (PDI)- Dynamic Light Scattering (DLS) NU-USC Biomedical Resource Core Nanocarrier/micelles characterization: RNA incorporation - Gel Electrophoresis NU-USC Biomedical Resource Core Nanocarrier/micelles characterization: RNA protection against nucleases - Gel Electrophoresis NU-USC Biomedical Resource Core Nanocarrier/micelles characterization: zeta potential - Dynamic Light Scattering (DLS) NU-USC Biomedical Resource Core Peptide purification NU-USC Biomedical Resource Core Peptide purification: High-Performance Liquid Chromatography (HPLC) NU-USC Biomedical Resource Core Peptide purification: Matrix-Assisted Laser Desorption/Ionization (MALDI) NU-USC Biomedical Resource Core Purified peptide conjugation with DSPE-PEG for micelles synthesis NU-USC Biomedical Resource Core Purified peptide conjugation with DSPE-PEG purification: High-Performance Liquid Chromoatography (HPLC) NU-USC Biomedical Resource Core Purified peptide conjugation with DSPE-PEG purification: Matrix-Assisted Laser Desorption/Ionization (MALDI) NU-USC Biomedical Resource Core Quantification of drug encapsulation in nanocarriers by HPLC NU-USC Biomedical Resource Core siRNA conjugation with DSPE-PEG NU-USC Biomedical Resource Core HPLC-Fl - ATP Metabolome Pittsburgh Model Systems Core Purine Metabolome by UPLC-MS/MS Pittsburgh Model Systems Core Epithelial transport in Ussing chambers Pittsburgh Physiology Core In vitro microperfusion of isolated tubules for functional fluorescence assays of single cell function Pittsburgh Physiology Core In vitro microperfusion of isolated tubules for measurement of transepithelial ion and solute transport Pittsburgh Physiology Core Isolation of single nephron segments for immunofluorescence, enzyme assays, RNA and protein isolation Pittsburgh Physiology Core Isolation of Xenopus laevis oocytes Pittsburgh Physiology Core Oocyte two-electrode voltage clamp Pittsburgh Physiology Core Patch-clamp analyses Pittsburgh Physiology Core Quantification of surface protein expression Pittsburgh Physiology Core AKI Biological Sample Repository UAB-UCSD Clinical Core Bulk RNA seq - from Fastq to analyses UAB-UCSD Pre-Clinical Core Bulk RNA seq - sample to analyses UAB-UCSD Pre-Clinical Core LC-MS/MS Creatinine determination (per test) UAB-UCSD Pre-Clinical Core Use of a microsurgical workstation (per hour) UAB-UCSD Pre-Clinical Core BCA Assay for normalization of fatty acid oxidation data WUSTL Metabolism Core Branched chain amino acid oxidation in tissue ex vivo using 14C-KIVA WUSTL Metabolism Core Cell quantification with Biotek Lionheart (to normalize Seahorse data) WUSTL Metabolism Core Fatty acid oxidation in tissue ex vivo using 3H-palmitate WUSTL Metabolism Core Glucose oxidation in tissue ex vivo using 14C-pyruvate WUSTL Metabolism Core Placement of jugular catheter for administration of stable isotopes WUSTL Metabolism Core Seahorse bioflux analyzer (XF24/XF96) to measure glucose oxidation in primary proximal tubule cells WUSTL Metabolism Core Seahorse bioflux analyzer (XF24/XF96) to measure glutamine oxidation in primary proximal tubule cells WUSTL Metabolism Core Seahorse bioflux analyzer (XF24/XF96) to measure glycolysis (i.e. extracellular acidification rate) in primary proximal tubule cells WUSTL Metabolism Core Seahorse bioflux analyzer (XF24/XF96) to measure mitochondrial oxidative respiration in primary proximal tubule cells WUSTL Metabolism Core Seahorse bioflux analyzer (XF24/XF96) to measure palmitate (fatty acid) oxidation in primary proximal tubule cells WUSTL Metabolism Core Stable isotope flux studies- proximal tubules: 13C-glucose WUSTL Metabolism Core Stable isotope flux studies- proximal tubules: 13C-glutamine WUSTL Metabolism Core Stable isotope flux studies- proximal tubules: 13C-palmitate WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-glucose WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-glutamine WUSTL Metabolism Core Stable isotope tracing studies in vivo: 13C-palmitate and 13C-oleate WUSTL Metabolism Core Stable isotope tracing studies in vivo: 15N-glutamine WUSTL Metabolism Core Stable isotope tracing studies in vivo: branched chain amino acids WUSTL Metabolism Core Untargeted metabolomics - lipids WUSTL Metabolism Core Untargeted metabolomics - water soluble WUSTL Metabolism Core Analysis of variant protein trafficking in cultured cells WUSTL Variant Validation Core Analysis of variant UMOD protein trafficking in cultured cells WUSTL Variant Validation Core Analysis of VUS in non-COL4A genes through design and implementation of tailored laboratory assays WUSTL Variant Validation Core COL4A3 intronic VUS analysis by minigene RNA splicing assays using transfected cells WUSTL Variant Validation Core COL4A3 VUS (missense) analysis by N-terminal split nano-Luciferase collagen IV assembly and secretion assays in transfected cells WUSTL Variant Validation Core COL4A3 VUS (missense) analysis by split C-terminal nano-Luciferase collagen IV assembly and secretion assays using transfected cells WUSTL Variant Validation Core COL4A3 VUS analysis by high resolution quantitative immunofluorescence imaging in paraffin sections of kidney biopsies WUSTL Variant Validation Core COL4A4 intronic VUS analysis by minigene RNA splicing assays using transfected cells WUSTL Variant Validation Core COL4A4 VUS (missense) analysis by N-terminal split nano-Luciferase collagen IV assembly and secretion assays in transfected cells WUSTL Variant Validation Core COL4A4 VUS (missense) analysis by split C-terminal nano-Luciferase collagen IV assembly and secretion assays using transfected cells WUSTL Variant Validation Core COL4A4 VUS analysis by high resolution quantitative immunofluorescence imaging in paraffin sections of kidney biopsies WUSTL Variant Validation Core COL4A5 intronic VUS analysis by minigene RNA splicing assays using transfected cells WUSTL Variant Validation Core COL4A5 VUS (missense) analysis by N-terminal split nano-Luciferase collagen IV assembly and secretion assays in transfected cells WUSTL Variant Validation Core COL4A5 VUS (missense) analysis by split C-terminal nano-Luciferase collagen IV assembly and secretion assays using transfected cells WUSTL Variant Validation Core COL4A5 VUS analysis by high resolution quantitative immunofluorescence imaging in paraffin sections of kidney biopsies WUSTL Variant Validation Core Intronic non-COL4A gene VUS analysis by minigene RNA splicing assays using transfected cells WUSTL Variant Validation Core -
Methods Development
Methods development in microscopy and molecular imaging Indiana University Intravital core/Molecular Imaging Resource cores Metabolomics driven analysis workflow Michigan Applied Systems Biology Core Spatial metabolomics service (sample process and data analysis) Michigan Applied Systems Biology Core Transcript driven data-mining workflow - Single cell RNA Sequencing analysis pipeline Michigan Applied Systems Biology Core Transcript driven data-mining workflow - Streamlined self-organizing map (SOM) to identify functional subgroups Michigan Applied Systems Biology Core Transcript driven data-mining workflow - Weighted Correlation Network Analysis (WGCNA) to identify co-expressed molecular elements within datasets Michigan Applied Systems Biology Core Design of epidemiological, clinical studies or clinical trials UAB-UCSD Clinical Core Forms, manual of operations, and/or instrument development UAB-UCSD Clinical Core Methodologies and Applications of Artificial Intelligence and Digital Health UAB-UCSD Clinical Core Research Resource Development (insert link for Development Core) UAB-UCSD Resource Development Core Design of assays for other protein trafficking in cultured cells WUSTL Variant Validation Core -
Microscopy/Imaging Services
Characterization of transgenic rodents expressing fluorescently tagged proteins in the kidney Indiana University Intravital Microscopy Resource Core Fluorescence lifetime imaging of endogenous and exogenous fluorophores in the kidney Indiana University Intravital Microscopy Resource Core Intravital Imaging: Apoptosis and necrosis Indiana University Intravital Microscopy Resource Core Intravital Imaging: Cellular oxidative stress Indiana University Intravital Microscopy Resource Core Intravital Imaging: Characterization of in vivo fluorescent probe delivery to the kidney Indiana University Intravital Microscopy Resource Core Intravital Imaging: Glomerular Permeability Indiana University Intravital Microscopy Resource Core Intravital Imaging: Microvascular flow Indiana University Intravital Microscopy Resource Core Intravital Imaging: Microvascular permeability Indiana University Intravital Microscopy Resource Core Intravital Imaging: Mitochondrial function Indiana University Intravital Microscopy Resource Core Intravital Imaging: Proximal tubule endocytosis Indiana University Intravital Microscopy Resource Core 3-dimensional confocal tissue cytometry of kidney tissue Indiana University Molecular Imaging Resource Core CODEX multiplexed cytometry of mouse and human kidney tissue, other tissues/organs Indiana University Molecular Imaging Resource Core In situ sequencing of mouse and human kidney tissue (Xenium) Indiana University Molecular Imaging Resource Core Spatial Transcriptomics of mouse and human kidney tissue (Visium SD, Visium FFPE) Indiana University Molecular Imaging Resource Core Images w/ deep phenotyping Johns Hopkins Biomedical Resource Core (C-DSSL) Confocal microscopy - Laser scanning confocal microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Confocal microscopy - Spinning disk confocal microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Confocal microscropy - Fluorescent lifetime imaging Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Confocal microscropy - Fluorescent recovery after photobleaching Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Confocal microscropy - Ribbon scanning confocal microscopy for cleared organs/tissues Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - CLEM Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Freeze fracture microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Freeze substitution Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Immuno-Electron microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Negative staining Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Platinum replica electron microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Scanning electron microscopy (field-emission) Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Transmission electron microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Ultramicrotomy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Electron Microscopy - Ultrathin cryo EM Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Image Analysis - Imaris Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Image Analysis - Leica LASX Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Image Analysis - Metamorph Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Image Analysis - NIH image/FIJI Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Image Analysis - NIS elements Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Image Analysis - Photoshop Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core iPOL imaging Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Live-cell microscopy - Fluorescent lifetime imaging Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Live-cell microscopy - Fluorescent recovery after photobleaching Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Live-cell microscopy - Incubator microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Live-cell microscopy - Lattice light sheet microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Live-cell microscopy - Spinning disk confocal microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Live-cell microscopy -Microinjection Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Meso-Spim light sheet microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Multiphoton microscopy - Fluorescent lifetime imaging Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Multiphoton microscopy - Fluorescent recovery after photobleaching Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Multiphoton microscopy - Intravital imaging Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Multiphoton microscopy - Multiphoton confocal microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Standard light microscopy - Brightfield, darkfield, epifluorescence, DIC Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Standard light microscopy - Macro (stereo) imaging microscopy Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Super-resolution microscopy - AX/NSPARC Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Super-resolution microscopy - CREST Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Super-resolution microscopy - STED Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Super-resolution microscopy - STORM Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core TRIF Microscopy - TIRF imaging acquisition and analysis Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Whole slide scanning - brightfield and epifluorescence Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Functional imaging analyses Pittsburgh Physiology Core Functional renal imaging (tracer dependent, per hour) UAB-UCSD Pre-Clinical Core Image Analysis UAB-UCSD Pre-Clinical Core Nuclear imaging (Gamma camera, SPECT/CT, PET/CT) (per hour) UAB-UCSD Pre-Clinical Core Optical Imaging (Bioluminescence, Fluorescence) (per hour) UAB-UCSD Pre-Clinical Core Structural and metabolic imaging (US, MRI, microCT) (per hour) UAB-UCSD Pre-Clinical Core -
Project Consultation
AKI study design and planning for imaging studies Indiana University Intravital core/Molecular Imaging Resource cores CKD study design and planning for imaging studies Indiana University Intravital core/Molecular Imaging Resource cores Project consultation for imaging studies Indiana University Intravital core/Molecular Imaging Resource cores Initial project consultation to select appropriate Basic Science Dietary and Social Stressor Laboratory resources Johns Hopkins Biomedical Resource Core (B-DSSL) Study design, Basic Science Dietary and Social Stressor Laboratory Johns Hopkins Biomedical Resource Core (B-DSSL) Consultation on design and features of translational studies in the community Johns Hopkins Biomedical Resource Core (C-DSSL) Initial project consultation to select appropriate Clinical Science Dietary and Social Stressor Laboratory resources Johns Hopkins Biomedical Resource Core (C-DSSL) Study design consultation and data and biobanked specimens from studies on behavioral interventions for lifestyle changes Johns Hopkins Biomedical Resource Core (C-DSSL) Study design, Clinical Science Dietary and Social Stressor Laboratory Johns Hopkins Biomedical Resource Core (C-DSSL) Shared Data Mining Services - Initial study design consultation Michigan Applied Systems Biology Core Applications of immunomagnetic cell sorting (LEAPFROG Platform) NU-USC Biomedical Resource Core Designing in vitro studies NU-USC Biomedical Resource Core Designing in vivo studies NU-USC Biomedical Resource Core Feasibility assessment NU-USC Biomedical Resource Core Identifying pathway for therapy NU-USC Biomedical Resource Core Identifying targeting peptides NU-USC Biomedical Resource Core In Vivo Micelles Therapeutic Efficacy : Hematoxylin and Eosin (H&E) Imaging NU-USC Biomedical Resource Core In Vivo Therapeutic Efficacy: NU-USC Biomedical Resource Core In Vivo Therapeutic Efficacy: Guidance NU-USC Biomedical Resource Core NW Background search NU-USC Biomedical Resource Core NW Grant proposal assistance NU-USC Biomedical Resource Core Consultation for development of new approaches to study the kidney and lower urinary tract using morphological tools Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core AKI Clinical Study Initiation and Conduct UAB-UCSD Clinical Core AKI Epidemiological Study Initiation and Conduct UAB-UCSD Clinical Core AKI Study Planning and Design UAB-UCSD Clinical Core Data Safety and Monitoring UAB-UCSD Clinical Core Bulk RNA seq UAB-UCSD Pre-Clinical Core Choosing the right metabolic assay WUSTL Metabolism Core High resolution respirometry (Oroboros) WUSTL Metabolism Core Metabolomics and stable isotope flux study- Zoom discussion with Patti lab over experimental design WUSTL Metabolism Core Choosing an assay for COL4A3 VUS analysis WUSTL Variant Validation Core Choosing an assay for COL4A4 VUS analysis WUSTL Variant Validation Core Choosing an assay for COL4A5 VUS analysis WUSTL Variant Validation Core Choosing an assay for non-COL4A gene VUS analysis WUSTL Variant Validation Core -
Publication Support
Manuscript development/publication support Indiana University Intravital core/Molecular Imaging Resource cores Hopkins Manuscript development Johns Hopkins Biomedical Resources Core Michigan Manuscript development Michigan All cores NU-USC Manuscript assistance NU-USC Biomedical Resource Core NU-USC Publication Assistance NU-USC Biomedical Resource Core Manuscript development Pittsburgh All Cores Assistance with manuscript development or publication support UAB-UCSD All Cores Assistance with manuscript development or publication support WUSTL Metabolism Core -
Training/Development
Training in Digital Image Analysis Indiana University Intravital core/Molecular Imaging Resource cores Training in Intravital microscopy Indiana University Intravital Resource core Isolated Nephron segment Dissection Johns Hopkins Biomedical Resource Core (B-DSSL) Kidney Slice Preparation Johns Hopkins Biomedical Resource Core (B-DSSL) Use of injection moulded microfluidic chips for cell sorting (LEAPFROG platform) NU-USC Resource Development Core Training in live-cell and intravital approaches to study the biology/pathobiology of kidney and bladder tissues Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Training in quantitative image analysis Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Training in sample preparation of kidney and bladder tissues for light and electron microscopic analysis Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Training in specialized sample preparation to perform labeling and analysis of cleared organs and tissues Pittsburgh Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core Nephron segment microdissection/isolation and microperfusion training Pittsburgh Physiology Core Patch-clamp training Pittsburgh Physiology Core Training on use of microinjectors Pittsburgh Physiology Core Training on use of micropipette pullers and microforges to craft micropipettes for patch-clamp and in vitro microperfusion Pittsburgh Physiology Core Two-electrode voltage clamp training Pittsburgh Physiology Core Xenopus laevis technologies training Pittsburgh Physiology Core Bulk RNA seq training (per day) UAB-UCSD Pre-Clinical Core Training (per hour/per day) UAB-UCSD Pre-Clinical Core Training in the performance of collagen IV heterotrimerization assays using split nanlLuciferase tagged COL4A chains WUSTL Variant Validation Core