The George M. O’Brien Kidney Resource Alliance (OKRA) provides over 200 specialized resources, tools, technologies, services, and expertise beyond those typically available in an individual lab or institutional core. If you are interested in requesting any of the resources below, please send an email with the following information to the OKRA National Coordinating Center at
- Principal Investigator Name
- Primary Institution
- Contact Email
- Early-stage investigator status (use this link to determine your designation)
- Project Title
- Funding Agency
- Resource(s) being requested/Resource Center
Online request form is coming soon!
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Animal Models/Procedures
Humanized mouse diet protocols
Biomedical Resource Core (B-DSSL)
Comprehensive mouse kidney phenotyping for diet and stress mechanisms
Biomedical Resource Core (B-DSSL)
Indirect calorimetry and Lipid and carbohydrate metabolism phenotyping
Biomedical Resource Core (B-DSSL)
Immunological manifestations of dietary and social stressors in the kidney
Biomedical Resource Core (B-DSSL)
Mouse biobank of dietary and social stress models
Biomedical Resource Core (B-DSSL)
Model systems studies to identify pathways shared cross-species
Applied Systems Biology Core
Yeast Models - Protein quality control (degradation, ubiquitylation) assays
Model Systems Core
Yeast Models - Protein trafficking assays (immunofluorescence, density centrifugation)
Model Systems Core
Yeast Models - Positive genetic selection assays
Model Systems Core
Yeast Models - Computational identification of functional variants
Model Systems Core
Yeast Models - Bioinformatic analysis of genotype-phenotype correlations (TopMed, Clinvar, UK BioBank)
Model Systems Core
Epithelial Models - Mammalian cell culture models (proximal tubule, collecting duct, polarized epithelia)
Model Systems Core
Epithelial Models - Organoid models to study nephron development
Model Systems Core
Epithelial Models - Organoid models to study kidney physiology
Model Systems Core
Epithelial Models - Organoid models to study kidney injury
Model Systems Core
Animal Models - Measurement of GFR and RBF
Model Systems Core
Animal Models - Metabolic cage studies, urine metabolites
Model Systems Core
Animal Models - Blood pressure telemetry
Model Systems Core
Animal Models - Cardiac Function (pressure-time parameters)
Model Systems Core
Animal Models - Real-time super-resolution ultrasound of kidney microvasculature
Model Systems Core
Animal Models - Purine metabolomics
Model Systems Core
Animal Models - Mitochondrial / Bioenergetics (Seahorse)
Model Systems Core
Animal Models - Injury biomarker assessment
Model Systems Core
Disease Models: AKI (ischemia-reperfusion injury)
Model Systems Core
Disease Models: AKI (cisplatin nephrotoxicity)
Model Systems Core
Disease Models: AKI (gentamicin nephrotoxicity)
Model Systems Core
Disease Models: AKI (cecal ligation and puncture)
Model Systems Core
Disease Models: AKI (sickle cell crisis)
Model Systems Core
Disease Models: CKD (genetic models)
Model Systems Core
Disease Models: CKD (FSGS)
Model Systems Core
Disease Models: CKD (unilateral ureteral obstruction)
Model Systems Core
Disease Models: CKD (two kidney one clip hypertension)
Model Systems Core
Disease Models: CKD (chronic angiotensin II infusion)
Model Systems Core
Special Rodent Diets (low and high Na+ and K+)
Model Systems Core
Kidney ischemia/reperfusion (IRI)
Pre-Clinical Core
Unilateral Ureteral Obstruction
Pre-Clinical Core
Orthotopic kidney transplantation
Pre-Clinical Core
Tail vein injection or venipuncture (per animal)
Pre-Clinical Core
GFR (transcutaneous FITC-sinistrin) in rodents
Pre-Clinical Core
Cannulation (indwelling with injection port)
Pre-Clinical Core
Misc. surgical services involving body wall penetration (per hour)
Pre-Clinical Core
5/6th nephrectomy (both steps)
Pre-Clinical Core
Radio-telemetry determination of blood pressure
Pre-Clinical Core
Whole kidney clearance/oxygen consumption studies
Pre-Clinical Core
Tissue oxygen partial pressure determination in kidney
Pre-Clinical Core
Determination of GFR in awake rodents
Pre-Clinical Core
Micropuncture for nephron function and TG feedback
Pre-Clinical Core
Metabolic cage experiments in mice (per animal and week)
Pre-Clinical Core
Automated tail-cuff blood pressure determination (each, 6 days)
Pre-Clinical Core
Laser Capture Microdissection from frozen tissue (~70 pieces individually collected per sample; sample quality and amount appropriate for RNA-Seq or potentially proteomics)
Pre-Clinical Core
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Biostatistics/Bioinformatics
Biostatistical support
Biomedical Resource Core (C-DSSL)
Quantitative morphometric analyses of kidney biopsy samples
Applied Systems Biology Core
Multi-scalar data analysis and integration
Applied Systems Biology Core
Nephroseq - access/training/support
Data Analytic Services Core
TranSMART - access/training/support
Data Analytic Services Core
CELLxGENE and Vitessce - access/training/support
Data Analytic Services Core
Investigating biomarker association (gene, protein, metabolite level) in clinical existing cohorts through TranSMART instance
Data Analytic Services Core
Develop generalized workflow for integrative web-based analysis
Data Analytic Services Core
AKI Study Analysis and Interpretation
Clinical Core
Analysis of pilot data
Clinical Core
Sample size and/or power analysis
Clinical Core
Analyses of study results
Clinical Core
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Data Provisioning
Dietary assessment and Dietary data
Biomedical Resource Core
Large longitudinal cohort study data from racially and socioeconomically diverse populations
Biomedical Resource Core (C-DSSL)
Publicly available datasets
Biomedical Resource Core (C-DSSL)
EMR registries
Biomedical Resource Core (C-DSSL)
Genomics dataset w/ deep phenotyping
Biomedical Resource Core (C-DSSL)
Proteomics dataset w/ deep phenotyping
Biomedical Resource Core (C-DSSL)
Metabolomics dataset w/ deep phenotyping
Biomedical Resource Core (C-DSSL)
Clinical and molecular interactions in investigators’ data sets
Applied Systems Biology Core
Cloud-based multimodal databases for AKI and critical care nephrology research studies
Clinical Core
Database development
Clinical Core
Big data transformation & pipeline development
Clinical Core
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Laboratory Services
Stored biospecimen samples from completed studies
Biomedical Resource Core (C-DSSL)
Healthy volunteer samples for Reference Range
Biomedical Resource Core (C-DSSL)
Prospective remnant sample collection
Biomedical Resource Core (C-DSSL)
Biomarker measurement - Immunoassays
Biomedical Resource Core (C-DSSL)
Biomarker measurement - Clinical Chemistry
Biomedical Resource Core (C-DSSL)
Biorepository support - Processing aliquoting, short- and long-term sample storage
Biomedical Resource Core (C-DSSL)
Microbiome; germ-free sample banks
Biomedical Resource Core (B-DSSL)
Structured RNA-DNA sequence data integration
Applied Systems Biology Core
Synthesis and Attachment of Targeting Moieties
Biomedical Resource Core
Gene Therapy and Drug Loading into Nanocarriers
Biomedical Resource Core
Isolation of single nephron segments for immunofluorescence, enzyme assays, RNA and protein isolation
Physiology Core
In vitro microperfusion of isolated tubules for measurement of transepithelial ion and solute transport
Physiology Core
In vitro microperfusion of isolated tubules for functional fluorescence assays of single cell function
Physiology Core
Isolation of Xenopus laevis oocytes
Physiology Core
Oocyte two-electrode voltage clamp
Physiology Core
Quantification of surface protein expression
Physiology Core
Patch-clamp analyses
Physiology Core
Epithelial transport in Ussing chambers
Physiology Core
AKI Biological Sample Repository
Clinical Core
Seahorse bioflux analyzer (XF24/XF96)
Metabolism Core
High resolution respirometry (Oroboros)
Metabolism Core
Substrate oxidation assays
Metabolism Core
Untargeted metabolomics
Metabolism Core
Stable isotope flux studies
Metabolism Core
COL4A3/A4/A5 VUS (missense) analysis by split nano-Luciferase collagen IV assembly and secretion assays using transfected cells
Variant Validation Core
COL4A3/A4/A5 VUS (intronic) analysis by RNA splicing assays using transfected cells
Variant Validation Core
COL4A3/A4/A5 VUS analysis by high resolution quantitative immunofluorescence imaging in paraffin sections of kidney biopsies
Variant Validation Core
COL4A3/A4/A5 VUS analysis in vivo by mouse gene editing
Variant Validation Core
Analysis of VUS in other genes through design and implementation of tailored laboratory assays
Variant Validation Core
LC-MS/MS Creatinine determination (per test)
Pre-Clinical Core
Use of a microsurgical workstation (per hour)
Pre-Clinical Core
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Methods Development
Transcript driven data-mining workflow - Single cell RNA Sequencing analysis pipeline
Applied Systems Biology Core
Transcript driven data-mining workflow - Streamlined self-organizing map (SOM) to identify functional subgroups
Applied Systems Biology Core
Transcript driven data-mining workflow - Weighted Correlation Network Analysis (WGCNA) to identify co-expressed molecular elements within datasets
Applied Systems Biology Core
Metabolomics driven analysis workflow
Applied Systems Biology Core
Design of epidemiological, clinical studies or clinical trials
Clinical Core
Forms, manual of operations, and/or instrument development
Clinical Core
Methodologies and Applications of Artificial Intelligence and Digital Health
Clinical Core
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Microscopy/Imaging Services
3-dimensional confocal tissue cytometry of kidney tissue
Molecular Imaging Resource Core
CODEX multiplexed cytometry of mouse and human kidney tissue
Molecular Imaging Resource Core
Spatial Transcriptomics of mouse and human kidney tissue (Visium)
Molecular Imaging Resource Core
In situ sequencing of mouse and human kidney tissue
Molecular Imaging Resource Core
Glomerular Permeability
Intravital Microscopy Resource Core
Proximal tubule endocytosis
Intravital Microscopy Resource Core
Microvascular flow
Intravital Microscopy Resource Core
Microvascular permeability
Intravital Microscopy Resource Core
Mitochondrial function
Intravital Microscopy Resource Core
Cellular oxidative stress
Intravital Microscopy Resource Core
Apoptosis and necrosis
Intravital Microscopy Resource Core
Characterization of in vivo fluorescent probe delivery to the kidney
Intravital Microscopy Resource Core
Characterization of transgenic rodents expressing fluorescently tagged proteins in the kidney
Intravital Microscopy Resource Core
Fluorescence lifetime imaging of endogenous and exogenous fluorophores in the kidney
Intravital Microscopy Resource Core
Functional imaging analyses
Physiology Core
Standard light microscopy - Brightfield, darkfield, epifluorescence, DIC
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Standard light microscopy - Macro (stereo) imaging microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Confocal microscopy - Laser scanning confocal microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Confocal microscopy - Spinning disk confocal microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Confocal microscropy - Ribbon scanning confocal microscopy for cleared organs/tissues
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Confocal microscropy - Fluorescent lifetime imaging
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Confocal microscropy - Fluorescent recovery after photobleaching
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Multiphoton microscopy - Multiphoton confocal microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Multiphoton microscopy - Fluorescent lifetime imaging
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Multiphoton microscopy - Fluorescent recovery after photobleaching
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Multiphoton microscopy - Intravital imaging
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
TRIF Microscopy - TIRF imaging acquisition and analysis
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Super-resolution microscopy - STED
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Super-resolution microscopy - CREST
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Super-resolution microscopy - STORM
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Super-resolution microscopy - AX/NSPARC
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Live-cell microscopy - Spinning disk confocal microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Live-cell microscopy - Fluorescent lifetime imaging
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Live-cell microscopy - Fluorescent recovery after photobleaching
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Live-cell microscopy - Lattice light sheet microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Live-cell microscopy - Incubator microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Live-cell microscopy -Microinjection
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Meso-Spim light sheet microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Whole slide scanning - brightfield and epifluorescence
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
iPOL imaging
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Scanning electron microscopy (field-emission)
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Transmission electron microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Freeze fracture microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Platinum replica electron microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Negative staining
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Freeze substitution
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Ultramicrotomy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Ultrathin cryo EM
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - CLEM
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Electron Microscopy - Immuno-Electron microscopy
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Image Analysis - Photoshop
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Image Analysis - Imaris
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Image Analysis - Metamorph
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Image Analysis - NIS elements
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Image Analysis - Leica LASX
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Image Analysis - NIH image/FIJI
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Images w/ deep phenotyping
Biomedical Resource Core (C-DSSL)
Functional renal imaging (tracer dependent, per hour)
Pre-Clinical Core
Structural and metabolic imaging (US, MRI, microCT) (per hour)
Pre-Clinical Core
Nuclear imaging (Gamma camera, SPECT/CT, PET/CT) (per hour)
Pre-Clinical Core
Optical Imaging (Bioluminescence, Fluorescence) (per hour)
Pre-Clinical Core
Image Analysis
Pre-Clinical Core
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Project Consultation
Clinical science study design
Biomedical Resource Core
Behavioral intervention studies
Biomedical Resource Core (C-DSSL)
Translational studies in the community
Biomedical Resource Core (C-DSSL)
Basic science study design
Biomedical Resource Core (B-DSSL)
Shared Data Mining Services - Initial study design consultation
Applied Systems Biology Core
Guidance in Molecular and Cell Target Selection
Biomedical Resource Core
Nanoparticle Carrier Selection Assistance
Biomedical Resource Core
Evaluating Targeting Fidelity In Vitro and In Vivo
Biomedical Resource Core
Consultation for development of new approaches to study the kidney and lower urinary tract using morphological tools
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
AKI Study Planning and Design
Clinical Core
AKI Clinical Study Initiation and Conduct
Clinical Core
AKI Epidemiological Study Initiation and Conduct
Clinical Core
Data Safety and Monitoring
Clinical Core
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Training/Development
Xenopus laevis technologies training
Physiology Core
Training on use of microinjectors
Physiology Core
Training on use of micropipette pullers and microforges to craft micropipettes for patch-clamp and in vitro microperfusion
Physiology Core
Two-electrode voltage clamp training
Physiology Core
Patch-clamp training
Physiology Core
Nephron segment microdissection/isolation and microperfusion training
Physiology Core
Training in sample preparation of kidney and bladder tissues for light and electron microscopic analysis
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training in specialized sample preparation to perform labeling and analysis of cleared organs and tissues
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training in quantitative image analysis
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training in live-cell and intravital approaches to study the biology/pathobiology of kidney and bladder tissues
Kidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training (per hour/per day)
Pre-Clinical Core